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Turkish Journal of Forensic Medicine

Turkish Journal of Forensic Medicine consisting of various original reseaches, case reports, letters, brief reports and reviews is published by the Council of Forensic Medicine as 3 issues per year. Membership or ordering particular issues are possible after filling in and sending the form.

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An LC-MS/MS method for determination of synthetic cannabinoids in human blood using bidirectional solid-phase extraction [J For Med]
J For Med. 2017; 31(3): 101-114 | DOI: 10.5505/adlitip.2017.30932  

An LC-MS/MS method for determination of synthetic cannabinoids in human blood using bidirectional solid-phase extraction

Oya Yeter
Council of Forensic Medicine, Chemistry Department, Istanbul, Turkey

INTRODUCTION: Objective: To present a sensitive and reliable liquid chromatography–tandem mass spectrometry (LC–MS/MS) method for determination of synthetic cannabinoids in blood samples is aimed in this study.
METHODS: Materials and Methods: The samples were prepared using a bidirectional solid phase extraction (BD-SPE) followed by injected to LC-MS/MS. A total of 57 synthetic cannabinoids and metabolites were seperated on an Agilent Poroshell 2.7μm (150 × 4.6 mm) column with gradient program and determined quantitatively. The method validation was done according to international guidelines.
RESULTS: Results: Limits of detection for all target analytes are 0.3 ng/mL or better, with only 0.5 mL of specimen used for analysis. The method is linear from 0.5 to 100 ng/mL with a correlation coefficient (R2) greater than 0.990. Recovery values were in the ranges of 54.0% and 115.0% with a mean of 87%. Matrix effects did not negatively affect analytical sensitivity and precision and accuracy were acceptable at any quality control level.
DISCUSSION AND CONCLUSION: Conclusion: The method allowing the determination of each analyte, even at low concentrations, can be used in the routine applications of forensic and clinical toxicology laboratories.

Keywords: Synthetic Cannabinoid, Forensic Toxicology, LC-MS/MS, Validation


Çift yönlü katı-faz ekstraksiyonu ve sıvı kromatografi-ardışık kütle spektrometresi ile kan örneklerinde sentetik kannabinoidlerin tayini

Oya Yeter
Adli Tıp Kurumu, Kimya İhtisas Dairesi, İstanbul,türkiye

GİRİŞ ve AMAÇ: Amaç: Son yıllarda kullanımı hızla artan sentetik kannabinoidlerin kan örneklerinde tayini için hassas ve güvenilir bir sıvı-kromatografisi ardışık kütle spektrometrik (LC-MS/MS) metot sunulması amaçlanmıştır.

YÖNTEM ve GEREÇLER: Materyal ve Metot: Kan örnekleri çift-yönlü katı faz ekstraksiyonu (BD-SPE) ile ekstrakte edildikten sonra LC-MS/MS’e enjekte edilmiştir. Toplam olarak 57 adet sentetik kannabinoid ve metabolitleri Poroshell 2,7μm (150×4.6mm) analitik kolonu ile gradiyent akış programı uygulanarak kantitatif olarak tayin edilmiştir. Metot validasyonu uluslararası rehberlere göre yapılmıştır.
BULGULAR: Bulgular: Sadece 0,5mL kan örneği kullanılarak, tespit (LOD) ve tayin (LOQ) sınırları 0,3 ve 0,5ng/mL veya daha düşük olarak belirlenmiştir. Metot 0,5-100ng/mL arasında en düşük 0,990 belirleme katsayısı ile lineerdir. Geri kazanım oranları %54- %115 arasında ve ortalama %87’dir. Matriks etkisi analiz hassasiyetini negatif olarak etkilememekte, kesinlik ve doğruluk kabul edilebilir seviyelerdedir.
TARTIŞMA ve SONUÇ: Sonuç: Her bir analitin düşük konsantrasyonlarda olsa bile tayinine imkan sağlayan metot adli ve klinik toksikoloji laboratuarlarının rutin uygulamalarında kullanılabilir.

Anahtar Kelimeler: Sentetik Kannabinoid, Adli Toksikoloji, LC-MS/MS, Metot Validasyonu.


Oya Yeter. An LC-MS/MS method for determination of synthetic cannabinoids in human blood using bidirectional solid-phase extraction. J For Med. 2017; 31(3): 101-114

Corresponding Author: Oya Yeter, Türkiye


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